Hotspot

ndhA

SSC · 113839-115529 · 1691 bp

Back to Pinguicula

Pi 0.027

MarkerSeek score 64.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 1691
variable_sites: 66
pi: 0.027
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GTGAAAGAAGCTGGGACGAA	TCCAACAGGCTATGGAAGGA	50.0	50.0	58.1	58.0	2184-2482 bp	1.0	83.5
ndhA_p2	GTGAAAGAAGCTGGGACGAA	CGAGTTGAAGGCATAGGCAT	50.0	50.0	58.1	58.0	2404-2702 bp	1.0	83.2
ndhA_p3	GTGAAAGAAGCTGGGACGAA	TGGCAAAAAGAGGGGGATTC	50.0	50.0	58.1	58.1	2257-2555 bp	1.0	83.2
ndhA_p4	GTGAAAGAAGCTGGGACGAA	CCGGGGTTGCTGAATATCAA	50.0	50.0	58.1	57.9	2457-2755 bp	1.0	83.0
ndhA_p5	GTGAAAGAAGCTGGGACGAA	AACCGGGGTTGCTGAATATC	50.0	50.0	58.1	57.9	2459-2757 bp	1.0	83.0