Hotspot

ccsA-ndhD

SSC · 110415-110653 · 239 bp

Back to Pinguicula

Pi 0.0339

MarkerSeek score 65.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 239
variable_sites: 12
pi: 0.0339
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TCATCAATTGTGGCGTCGAT	TGCCCGTACTAGGTATTGGT	45.0	50.0	58.0	57.8	408-435 bp	1.0	82.3
ccsA-ndhD_p2	GTTCATCAATTGTGGCGTCG	TGCCCGTACTAGGTATTGGT	50.0	50.0	58.1	57.8	410-437 bp	1.0	81.5
ccsA-ndhD_p3	TTCATCAATTGTGGCGTCGA	TGCCCGTACTAGGTATTGGT	45.0	50.0	58.2	57.8	409-436 bp	1.0	80.9
ccsA-ndhD_p4	AATTGTGGCGTCGATTGGAT	TGCCCGTACTAGGTATTGGT	45.0	50.0	58.2	57.8	403-430 bp	1.0	80.5
ccsA-ndhD_p5	ATTGTGGCGTCGATTGGATT	TGCCCGTACTAGGTATTGGT	45.0	50.0	58.2	57.8	402-429 bp	1.0	80.5