Hotspot

rpl32-trnL-UAG

SSC · 108691-109280 · 590 bp

Back to Pinguicula

Pi 0.0833

MarkerSeek score 85.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 590
variable_sites: 72
pi: 0.0833
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 85.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ACCGGGAATTCAAAAAGTTT	TCCTAAGAGCAGCGTGTCTA	35.0	50.0	53.9	57.9	689-715 bp	1.0	55.5
rpl32-trnL-UAG_p2	ACCGGGAATTCAAAAAGTTT	AGCAGCGTGTCTACCAATTT	35.0	45.0	53.9	57.8	682-708 bp	1.0	55.1
rpl32-trnL-UAG_p3	ACCGGGAATTCAAAAAGTTT	CCTAAGAGCAGCGTGTCTAC	35.0	55.0	53.9	57.8	688-714 bp	1.0	55.0
rpl32-trnL-UAG_p4	ACCGGGAATTCAAAAAGTTT	CTAAGAGCAGCGTGTCTACC	35.0	55.0	53.9	57.8	687-713 bp	1.0	55.0
rpl32-trnL-UAG_p5	ACCGGGAATTCAAAAAGTTT	GAGATGACATGCCGCTACTC	35.0	55.0	53.9	58.2	739-765 bp	1.0	54.2