Hotspot

petA-psbJ

LSC · 61373-62182 · 810 bp

Back to Pinguicula

Pi 0.04

MarkerSeek score 61.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 810
variable_sites: 46
pi: 0.04
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCGGATTTGGTCAAGGAGAT	ACACAATCAAACCCGAACGA	50.0	45.0	58.0	58.0	1333-1518 bp	1.0	84.3
petA-psbJ_p2	TCCTAATGTGGGCGGATTTG	ACACAATCAAACCCGAACGA	50.0	45.0	57.9	58.0	1344-1529 bp	1.0	84.2
petA-psbJ_p3	TTTATCCCGACGGAAGCAAG	ACACAATCAAACCCGAACGA	50.0	45.0	58.0	58.0	1558-1743 bp	1.0	84.0
petA-psbJ_p4	CGACGGAAGCAAGAGTAACA	ACACAATCAAACCCGAACGA	50.0	45.0	57.9	58.0	1551-1736 bp	1.0	83.7
petA-psbJ_p5	TCCGATCGTATTTCTCCCGA	ACACAATCAAACCCGAACGA	50.0	45.0	58.0	58.0	1778-1963 bp	1.0	83.5