Hotspot

trnF-GAA-ndhJ

LSC · 47723-48300 · 578 bp

Back to Pinguicula

Pi 0.0809

MarkerSeek score 68.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 578
variable_sites: 70
pi: 0.0809
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TGAAATACAAGATGCTCAGTGA	55.0	36.4	58.0	55.5	670-704 bp	1.0	51.9
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TGAAATACAAGATGCTCAGTGA	50.0	36.4	58.1	55.5	634-668 bp	1.0	51.5
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	TGAAATACAAGATGCTCAGTGA	55.0	36.4	57.9	55.5	658-692 bp	1.0	50.8
trnF-GAA-ndhJ_p4	GAATGGTCGGGATAGCTCAG	TGAAATACAAGATGCTCAGTGA	55.0	36.4	57.6	55.5	678-712 bp	1.0	48.3
trnF-GAA-ndhJ_p5	AATCCTCGTGTCACCAGTTC	TGAAATACAAGATGCTCAGTGA	50.0	36.4	57.5	55.5	637-671 bp	1.0	48.3