Hotspot

trnT-UGU-trnL-UAA

LSC · 46083-46719 · 637 bp

Back to Pinguicula

Pi 0.0368
MarkerSeek score 61.40
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
637
variable_sites
35
pi
0.0368
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 698-760 bp 1.0 81.1
trnT-UGU-trnL-UAA_p2 ATACCAAAAGCCGGCTATCG CCGTAGCGTCTACCGATTTC 50.0 55.0 58.1 58.2 749-811 bp 1.0 80.9
trnT-UGU-trnL-UAA_p3 GACTCGAACCGATGACCATC CCGTAGCGTCTACCGATTTC 55.0 55.0 58.2 58.2 729-791 bp 1.0 80.7
trnT-UGU-trnL-UAA_p4 GCGATGCTCTAACCTCTGAG CGTCTACCGATTTCGCCATA 55.0 50.0 57.9 57.6 692-754 bp 1.0 79.8
trnT-UGU-trnL-UAA_p5 ATACCAAAAGCCGGCTATCG CGTCTACCGATTTCGCCATA 50.0 50.0 58.1 57.6 743-805 bp 1.0 79.6