Hotspot

psbZ-trnG-UCC

LSC · 36116-36403 · 288 bp

Back to Pinguicula

Pi 0.043

MarkerSeek score 61.20

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 288
variable_sites: 18
pi: 0.043
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TTCCTGATGGTTGGTCAAGT	AATCGAACCCGCATCTTCTC	45.0	50.0	56.7	58.1	448-450 bp	1.0	73.6
psbZ-trnG-UCC_p2	TTCCTGATGGTTGGTCAAGT	GAATCGAACCCGCATCTTCT	45.0	50.0	56.7	58.1	449-451 bp	1.0	73.6
psbZ-trnG-UCC_p3	TTTCCTGATGGTTGGTCAAGT	AATCGAACCCGCATCTTCTC	42.9	50.0	57.4	58.1	449-451 bp	1.0	71.6
psbZ-trnG-UCC_p4	TTTCCTGATGGTTGGTCAAGT	GAATCGAACCCGCATCTTCT	42.9	50.0	57.4	58.1	450-452 bp	1.0	71.6
psbZ-trnG-UCC_p5	TTCCTGATGGTTGGTCAAGT	GAACCCGCATCTTCTCCTTG	45.0	55.0	56.7	58.6	444-446 bp	1.0	69.1