Hotspot

trnK-UUU-rps16

LSC · 4343-5060 · 718 bp

Back to Pinguicula

Pi 0.016
MarkerSeek score 60.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
718
variable_sites
17
pi
0.016
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGCACTTAAAAGCCGAGTA TCCTTGAGAAAGGTGCTCAAC 50.0 47.6 58.3 58.1 847-1032 bp 1.0 70.7
trnK-UUU-rps16_p2 TTCAAGCCGCACTTAAAAGC TCCTTGAGAAAGGTGCTCAAC 45.0 47.6 57.6 58.1 853-1038 bp 1.0 69.6
trnK-UUU-rps16_p3 CCGCACTTAAAAGCCGAGTA TCCTTGAGAAAGGTGCTCAA 50.0 45.0 58.3 56.7 847-1032 bp 1.0 69.3
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA TTCCTTGAGAAAGGTGCTCA 50.0 45.0 58.3 56.7 848-1033 bp 1.0 69.3
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA CCTTGAGAAAGGTGCTCAAC 50.0 50.0 58.3 56.6 846-1031 bp 1.0 69.0