Hotspot

trnH-GUG-psbA

LSC · 84-450 · 367 bp

Back to Pinguicula

Pi 0.0999

MarkerSeek score 57.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 367
variable_sites: 37
pi: 0.0999
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CAATCCACTGCCTTGATCCA	GAGCTTAGTTTCCGTCTGGG	50.0	55.0	57.9	58.0	971-1069 bp	1.0	86.5
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GAGTGGGAGCTTAGTTTCCG	50.0	55.0	57.9	58.0	977-1075 bp	1.0	86.5
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	GGAGCTTAGTTTCCGTCTGG	50.0	55.0	57.9	58.0	972-1070 bp	1.0	86.5
trnH-GUG-psbA_p4	CAATCCACTGCCTTGATCCA	GGGAGCTTAGTTTCCGTCTG	50.0	55.0	57.9	58.0	973-1071 bp	1.0	86.5
trnH-GUG-psbA_p5	AATCCACTGCCTTGATCCAC	GAGCTTAGTTTCCGTCTGGG	50.0	55.0	57.9	58.0	970-1068 bp	1.0	86.5