Hotspot

ycf1-trnR(ACG)

SSC · 138459-140132 · 1674 bp

Back to Pinellia

Pi 0.0552

MarkerSeek score 81.60

Primer available yes

Label ycf1-trnR(ACG)

Feature Metadata

label_name: ycf1-trnR(ACG)
feature_type: igs
region: SSC
length_bp: 1674
variable_sites: 169
pi: 0.0552
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnR(ACG)_p1	TTTTCTTTTCCACTCGGGGG	GGTGACGATACTGTAGGGGA	50.0	55.0	58.0	57.9	1808-2466 bp	1.0	89.7
ycf1-trnR(ACG)_p2	TTTTCTTTTCCACTCGGGGG	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	2140-2798 bp	1.0	89.0
ycf1-trnR(ACG)_p3	TTTTCTTTTCCACTCGGGGG	GTCAAGTGGAAGTGCAGTGA	50.0	50.0	58.0	58.0	2166-2824 bp	1.0	88.9
ycf1-trnR(ACG)_p4	TTTTCTTTTCCACTCGGGGG	GCTGAGGCATCCTAACAGAC	50.0	55.0	58.0	58.1	2138-2796 bp	1.0	88.9
ycf1-trnR(ACG)_p5	TCATTGCTCAAGGTGTGCTT	GGTGACGATACTGTAGGGGA	45.0	55.0	58.0	57.9	2249-2907 bp	1.0	88.9