Hotspot

rps15-ycf1

SSC · 132087-133472 · 1386 bp

Back to Pinellia

Pi 0.192

MarkerSeek score 78.00

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 1386
variable_sites: 472
pi: 0.192
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCCCAACGCGTTACATAAGG	AGCACTATGTTTGTGAGCGA	50.0	45.0	58.0	57.5	2043-2391 bp	0.8	80.3
rps15-ycf1_p2	ACGGACCCCTTGTTTTCTTC	AGCACTATGTTTGTGAGCGA	50.0	45.0	58.0	57.5	1520-1868 bp	0.8	80.2
rps15-ycf1_p3	GACGGACCCCTTGTTTTCTT	AGCACTATGTTTGTGAGCGA	50.0	45.0	58.0	57.5	1521-1869 bp	0.8	80.2
rps15-ycf1_p4	AGCTATGCGACTCAGCTCTA	AGCACTATGTTTGTGAGCGA	50.0	45.0	58.0	57.5	2167-2515 bp	0.8	80.1
rps15-ycf1_p5	TCTTGTAGCCGGTACAGTCA	AGCACTATGTTTGTGAGCGA	50.0	45.0	58.1	57.5	1852-2200 bp	0.8	79.7