Hotspot

ndhG-ndhI

SSC · 126883-127699 · 817 bp

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Pi 0.141

MarkerSeek score 78.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 817
variable_sites: 209
pi: 0.141
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AAGTAACCCCATCTCCCACA	TCAATCACATCGGAGCGTTT	50.0	45.0	57.9	57.9	1102-1676 bp	1.0	90.8
ndhG-ndhI_p2	CAGCGAAGTAACCCCATCTC	TCAATCACATCGGAGCGTTT	55.0	45.0	58.1	57.9	1107-1681 bp	1.0	90.8
ndhG-ndhI_p3	CCATCTCCCACAGTCCAAAG	TCAATCACATCGGAGCGTTT	55.0	45.0	57.9	57.9	1094-1668 bp	1.0	90.3
ndhG-ndhI_p4	AAGTAACCCCATCTCCCACA	CGGGGTCGAATCCATTTTGA	50.0	50.0	57.9	58.3	1081-1655 bp	1.0	89.5
ndhG-ndhI_p5	CAGCGAAGTAACCCCATCTC	CGGGGTCGAATCCATTTTGA	55.0	50.0	58.1	58.3	1086-1660 bp	1.0	89.5