Hotspot

trnN(GUU)-ndhF

SSC · 115352-117284 · 1933 bp

Back to Pinellia

Pi 0.156

MarkerSeek score 76.60

Primer available yes

Label trnN(GUU)-ndhF

Feature Metadata

label_name: trnN(GUU)-ndhF
feature_type: igs
region: SSC
length_bp: 1933
variable_sites: 489
pi: 0.156
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN(GUU)-ndhF_p1	AGGATTCGAACCTACGACCA	TGGTTGGCTCCATCGAAAAA	50.0	45.0	58.2	58.0	1206-2550 bp	0.8	82.7
trnN(GUU)-ndhF_p2	ATCATGATCGGGATAGCGGA	AGTTTCTTTGCGGGTGAAGT	50.0	45.0	58.1	57.9	1374-2729 bp	0.8	82.4
trnN(GUU)-ndhF_p3	ACTCCCCGTAACTCTTCCTC	TGGTTGGCTCCATCGAAAAA	55.0	45.0	58.2	58.0	2527-2898 bp	0.4	64.8
trnN(GUU)-ndhF_p4	CTCCCCGTAACTCTTCCTCA	TGGTTGGCTCCATCGAAAAA	55.0	45.0	58.2	58.0	2526-2897 bp	0.4	64.8
trnN(GUU)-ndhF_p5	TCCCCGTAACTCTTCCTCAG	TGGTTGGCTCCATCGAAAAA	55.0	45.0	58.2	58.0	2525-2896 bp	0.4	64.8