Hotspot

petA-psbJ

LSC · 68332-69284 · 953 bp

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Pi 0.0642

MarkerSeek score 83.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 953
variable_sites: 119
pi: 0.0642
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TATACCTCCAGGACCGGAAC	GAGAACCGACAAGGGATTCC	55.0	55.0	57.9	58.0	1744-1874 bp	1.0	89.0
petA-psbJ_p2	CCGATGGGAGCAAGAGTAAC	TGGACTAGCTGTACCTACCG	55.0	55.0	58.1	58.0	1736-1866 bp	1.0	88.9
petA-psbJ_p3	CCGATGGGAGCAAGAGTAAC	GGACTAGCTGTACCTACCGT	55.0	55.0	58.1	58.0	1735-1865 bp	1.0	88.9
petA-psbJ_p4	CCGATGGGAGCAAGAGTAAC	GAGAACCGACAAGGGATTCC	55.0	55.0	58.1	58.0	1880-2010 bp	1.0	88.8
petA-psbJ_p5	TAGGTCCTGTTCCTGGTCAG	GAGAACCGACAAGGGATTCC	55.0	55.0	58.1	58.0	2018-2148 bp	1.0	88.4