Hotspot

trnT(UGU)-trnL(UAA)

LSC · 50012-51231 · 1220 bp

Back to Pinellia

Pi 0.0836

MarkerSeek score 95.40

Primer available yes

Label trnT(UGU)-trnL(UAA)

Feature Metadata

label_name: trnT(UGU)-trnL(UAA)
feature_type: igs
region: LSC
length_bp: 1220
variable_sites: 194
pi: 0.0836
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 95.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT(UGU)-trnL(UAA)_p1	GGAAAAAGCCAGCTATCGGA	TCCATACCAAGGCTCAATGC	50.0	50.0	58.0	57.9	1267-1548 bp	1.0	91.2
trnT(UGU)-trnL(UAA)_p2	AGGGAAAAAGCCAGCTATCG	TCCATACCAAGGCTCAATGC	50.0	50.0	58.0	57.9	1269-1550 bp	1.0	91.2
trnT(UGU)-trnL(UAA)_p3	GGAAAAAGCCAGCTATCGGA	ACCAAGGCTCAATGCAATCA	50.0	45.0	58.0	58.1	1262-1543 bp	1.0	91.1
trnT(UGU)-trnL(UAA)_p4	AGGGAAAAAGCCAGCTATCG	ACCAAGGCTCAATGCAATCA	50.0	45.0	58.0	58.1	1264-1545 bp	1.0	91.1
trnT(UGU)-trnL(UAA)_p5	GGAAAAAGCCAGCTATCGGA	GCGTCTACCTATTTCGCCAT	50.0	50.0	58.0	57.8	1234-1515 bp	1.0	90.5