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psbC

LSC · 34704-36125 · 1422 bp

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Pi 0.000703

MarkerSeek score 38.80

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 2
pi: 0.000703
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	AGGGTATTCGTGCTTGGATG	TAGCCGAGCCAGAACAGAAA	50.0	50.0	57.7	59.0	1513 bp	1.0	69.2
psbC_p2	AGGGTATTCGTGCTTGGATG	GCCGAGCCAGAACAGAAAAA	50.0	50.0	57.7	59.0	1511 bp	1.0	69.1
psbC_p3	GAGGGTATTCGTGCTTGGAT	TAGCCGAGCCAGAACAGAAA	50.0	50.0	57.4	59.0	1514 bp	1.0	66.9
psbC_p4	AGGGTATTCGTGCTTGGATG	TAGCCGAGCCAGAACAGAA	50.0	52.6	57.7	58.3	1513 bp	1.0	66.8
psbC_p5	GAGGGTATTCGTGCTTGGAT	GCCGAGCCAGAACAGAAAAA	50.0	50.0	57.4	59.0	1512 bp	1.0	66.7