Hotspot

psbA

LSC · 56-1117 · 1062 bp

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Pi 0.0122

MarkerSeek score 55.80

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 26
pi: 0.0122
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TCAACGATTCATATACACTAAGACA	GTGCTTGGGAGTCCTTGAAA	32.0	50.0	55.9	58.0	1136 bp	1.0	42.5
psbA_p2	CAACGATTCATATACACTAAGACA	GTGCTTGGGAGTCCTTGAAA	33.3	50.0	54.6	58.0	1135 bp	1.0	42.5
psbA_p3	TCAACGATTCATATACACTAAGACAA	GTGCTTGGGAGTCCTTGAAA	30.8	50.0	56.6	58.0	1136 bp	1.0	42.5
psbA_p4	TTCAACGATTCATATACACTAAGACA	GTGCTTGGGAGTCCTTGAAA	30.8	50.0	56.6	58.0	1137 bp	1.0	42.5
psbA_p5	TCAACGATTCATATACACTAAGACA	AATACGTGTGCTTGGGAGTC	32.0	50.0	55.9	57.9	1143 bp	1.0	42.4