Hotspot

rpl22-rps19

LSC · 86227-86348 · 122 bp

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Pi 0.0273

MarkerSeek score 75.10

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 6
pi: 0.0273
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGTCCGCGAATCTGATCAAT	TACTTTCGCGAGACATGCAA	50.0	45.0	58.5	57.9	284-307 bp	1.0	78.3
rpl22-rps19_p2	CGTCCGCGAATCTGATCAAT	TTACTTTCGCGAGACATGCA	50.0	45.0	58.5	57.9	285-308 bp	1.0	78.3
rpl22-rps19_p3	CGTCCGCGAATCTGATCAAT	GAGAATTCGCGCCTACTCTT	50.0	50.0	58.5	57.8	303-326 bp	1.0	77.0
rpl22-rps19_p4	CGTCCGCGAATCTGATCAAT	CAAATTGGGAGAATTCGCGC	50.0	50.0	58.5	58.5	311-334 bp	1.0	75.0
rpl22-rps19_p5	CGTCCGCGAATCTGATCAAT	TTCGCGAGACATGCAAGAAA	50.0	45.0	58.5	58.5	280-303 bp	1.0	75.0