Hotspot

atpF-atpH

LSC · 13738-14277 · 540 bp

Back to Pinanga

Pi 0.00377

MarkerSeek score 61.60

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 540
variable_sites: 4
pi: 0.00377
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	ATTTATGGACTGGTCGTGGC	50.0	50.0	57.9	58.0	652-663 bp	1.0	80.6
atpF-atpH_p2	CAGTGACCCAAGGAAAGGAA	ATTTATGGACTGGTCGTGGC	50.0	50.0	57.3	58.0	624-635 bp	1.0	76.3
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	GCGTTAGCACTTTTGTTTGC	50.0	45.0	57.9	57.1	634-645 bp	1.0	73.7
atpF-atpH_p4	CAGTGACCCAAGGAAAGGAAA	ATTTATGGACTGGTCGTGGC	47.6	50.0	58.1	58.0	624-635 bp	1.0	73.2
atpF-atpH_p5	GTGACCCAAGGAAAGGAAAGA	ATTTATGGACTGGTCGTGGC	47.6	50.0	57.8	58.0	622-633 bp	1.0	71.8