Hotspot

ndhC-trnV-UAC

LSC · 57063-57803 · 741 bp

Back to Pimpinella

Pi 0.0699

MarkerSeek score 78.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 741
variable_sites: 151
pi: 0.0699
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	31.8	55.0	53.9	58.1	710-1232 bp	1.0	52.0
ndhC-trnV-UAC_p2	ATCATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	30.4	55.0	54.5	58.1	711-1233 bp	1.0	52.0
ndhC-trnV-UAC_p3	TCATATTCGTAAAGCAGAAACAT	CCGAGAAAGTCTACGGTTCG	30.4	55.0	54.5	58.1	710-1232 bp	1.0	52.0
ndhC-trnV-UAC_p4	TCATATTCGTAAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	31.8	55.0	53.9	58.7	695-1217 bp	1.0	52.0
ndhC-trnV-UAC_p5	CATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	33.3	55.0	52.3	58.1	709-1231 bp	1.0	52.0