Hotspot

trnT-GGU-psbD

LSC · 37812-39227 · 1416 bp

Back to Pimpinella

Pi 0.0389

MarkerSeek score 72.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1416
variable_sites: 182
pi: 0.0389
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AGTCATCGGTTCAAATCCGG	AAACTAGGCGCAAAGAACCA	50.0	45.0	58.0	58.0	1966-1989 bp	0.230769	51.4
trnT-GGU-psbD_p2	AGTCATCGGTTCAAATCCGG	ACCAAAACTAGGCGCAAAGA	50.0	45.0	58.0	58.0	1970-1993 bp	0.230769	51.3
trnT-GGU-psbD_p3	CCGCTACAAAGGTACTGCAT	AAACTAGGCGCAAAGAACCA	50.0	45.0	58.0	58.0	2138-2161 bp	0.115385	44.6
trnT-GGU-psbD_p4	CCGCTACAAAGGTACTGCAT	ACCAAAACTAGGCGCAAAGA	50.0	45.0	58.0	58.0	2142-2165 bp	0.115385	44.6
trnT-GGU-psbD_p5	AAGAATAAGCCACTAGCCGC	AAACTAGGCGCAAAGAACCA	50.0	45.0	58.0	58.0	2154-2177 bp	0.115385	44.4