Hotspot

trnC-GCA-petN

LSC · 34105-34800 · 696 bp

Back to Pimpinella

Pi 0.0429
MarkerSeek score 80.40
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
696
variable_sites
94
pi
0.0429
unique_haplotype_count
23
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 568-764 bp 1.0 90.1
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG CGTCCCCATACTACGAGTGA 50.0 55.0 58.1 58.3 618-814 bp 0.961538 87.2
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG TCGTCCCCATACTACGAGTG 50.0 55.0 58.1 58.3 619-815 bp 0.961538 87.2
trnC-GCA-petN_p4 TTTCCCCAGTTCAAATCCGG TCTAGAGTCCACTTCGTCCC 50.0 55.0 58.1 57.9 816 bp 0.076923 41.4
trnC-GCA-petN_p5 GGGGGACTGCAAATCCTTTT TCTAGAGTCCACTTCGTCCC 50.0 55.0 58.4 57.9 834 bp 0.076923 39.2