Hotspot

rpoB-trnC-GCA

LSC · 32781-34033 · 1253 bp

Back to Pimpinella

Pi 0.0433

MarkerSeek score 76.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1253
variable_sites: 178
pi: 0.0433
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGAGCCCATATCCTTGCTTT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1784-1851 bp	1.0	90.7
rpoB-trnC-GCA_p2	CCGCCTACACAAGCAAATTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1976-2043 bp	1.0	90.5
rpoB-trnC-GCA_p3	CGAGCCCATATCCTTGCTTT	CTTACCACTCGGCCATATCG	50.0	55.0	58.0	57.9	1764-1811 bp	0.884615	84.7
rpoB-trnC-GCA_p4	CCGCCTACACAAGCAAATTG	CTTACCACTCGGCCATATCG	50.0	55.0	58.0	57.9	1956-2003 bp	0.884615	84.0
rpoB-trnC-GCA_p5	CCGCCTACACAAGCAAATTG	CGGAATTCAAGACTCAGCCA	50.0	50.0	58.0	57.9	2223-2291 bp	0.307692	59.5