Hotspot

atpH-atpI

LSC · 19263-20404 · 1142 bp

Back to Pimpinella

Pi 0.0446

MarkerSeek score 74.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1142
variable_sites: 168
pi: 0.0446
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TGCTTTGGCTTTACTCACGT	45.0	45.0	58.0	58.0	846-1537 bp	1.0	91.4
atpH-atpI_p2	CTCGCGATACCTTCTACAGC	TGCTTTGGCTTTACTCACGT	55.0	45.0	58.0	58.0	936-1627 bp	1.0	91.4
atpH-atpI_p3	CTTGGACTACGAACGGGAAG	TGCTTTGGCTTTACTCACGT	55.0	45.0	58.0	58.0	1253-1942 bp	0.884615	86.4
atpH-atpI_p4	CTTGGACTACGAACGGGAAG	TTCGGAACCCACAAACCATT	55.0	45.0	58.0	57.9	1494-2183 bp	0.884615	85.6
atpH-atpI_p5	TACTCATCCGCAAATCAGCC	TGCTTTGGCTTTACTCACGT	50.0	45.0	58.0	58.0	1208-1897 bp	0.230769	54.4