Hotspot

ycf1

SSC · 123640-128724 · 5085 bp

Back to Pilosella

Pi 0.00618

MarkerSeek score 35.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5085
variable_sites: 78
pi: 0.00618
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGAGAATTAGGATAGGGAAGAAGG	TGCTGAGACTAAAAACAAAAAGA	45.8	30.4	58.0	55.0	204 bp	1.0	42.4
ycf1_p2	CGAGAATTAGGATAGGGAAGAAGG	TGCTGAGACTAAAAACAAAAAGAA	45.8	29.2	58.0	55.7	204 bp	1.0	42.4
ycf1_p3	CGAGAATTAGGATAGGGAAGAAGG	TGAGACTAAAAACAAAAAGAATTGCC	45.8	30.8	58.0	57.6	201 bp	1.0	42.4
ycf1_p4	TCTCGAGAATTAGGATAGGGAAGA	TGCTGAGACTAAAAACAAAAAGA	41.7	30.4	57.8	55.0	207 bp	1.0	42.3
ycf1_p5	TCTCGAGAATTAGGATAGGGAAGA	TGCTGAGACTAAAAACAAAAAGAA	41.7	29.2	57.8	55.7	207 bp	1.0	42.3