Hotspot

petD

LSC · 77067-78254 · 1188 bp

Back to Pilosella

Pi 0.00303

MarkerSeek score 33.60

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1188
variable_sites: 9
pi: 0.00303
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	CAGTTCATCGATGGTCAGCA	CAGAATTGCCTCCCAGGATC	50.0	55.0	58.0	58.0	2169-2176 bp	1.0	80.8
petD_p2	TATCGTCCGACCGTTACTGA	CAGAATTGCCTCCCAGGATC	50.0	55.0	58.0	58.0	2248-2255 bp	1.0	80.7
petD_p3	CAGTTCATCGATGGTCAGCA	AGGGCAATCAACACATGGTT	50.0	45.0	58.0	58.0	2286-2287 bp	0.8	71.8
petD_p4	TATCGTCCGACCGTTACTGA	AGGGCAATCAACACATGGTT	50.0	45.0	58.0	58.0	2365-2366 bp	0.8	71.7
petD_p5	ACTATCGTCCGACCGTTACT	AGGGCAATCAACACATGGTT	50.0	45.0	58.0	58.0	2367-2368 bp	0.8	71.7