Hotspot

ccsA

SSC · 114311-115279 · 969 bp

Back to Pilosella

Pi 0.00165

MarkerSeek score 31.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 4
pi: 0.00165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	GTTTTTGATTCTGGACCGCG	50.0	50.0	58.0	58.0	1545-1556 bp	1.0	81.2
ccsA_p2	TGAAATCGGTAGACACGCTG	GTTTTTGATTCTGGACCGCG	50.0	50.0	58.0	58.0	1544-1555 bp	1.0	81.2
ccsA_p3	GTGAAATCGGTAGACACGCT	GCTTGCATTACCGGGTATGA	50.0	50.0	58.0	58.0	1750-1761 bp	1.0	81.0
ccsA_p4	TGAAATCGGTAGACACGCTG	GCTTGCATTACCGGGTATGA	50.0	50.0	58.0	58.0	1749-1760 bp	1.0	81.0
ccsA_p5	GTGAAATCGGTAGACACGCT	GCATTACCGGGTATGAGTGG	50.0	55.0	58.0	58.1	1746-1757 bp	1.0	80.3