Hotspot

ycf15-rps12

IRa · 138282-139264 · 983 bp

Back to Pilosella

Pi 0.000831

MarkerSeek score 53.70

Primer available yes

Label ycf15-rps12

Feature Metadata

label_name: ycf15-rps12
feature_type: igs
region: IRa
length_bp: 983
variable_sites: 2
pi: 0.000831
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-rps12_p1	GAAAGGACTCACTAAGCCGG	TGGCAACTTTACGTAAGGCA	55.0	45.0	58.0	57.7	1038-1058 bp	1.0	79.5
ycf15-rps12_p2	CACGGTACAATCCTCTTCCC	TGGCAACTTTACGTAAGGCA	55.0	45.0	57.7	57.7	1127-1147 bp	1.0	77.0
ycf15-rps12_p3	GAAAGGACTCACTAAGCCGG	ACGTAAGGCAGAGTTTGGTT	55.0	45.0	58.0	57.4	1028-1048 bp	1.0	76.5
ycf15-rps12_p4	TCACTAAGCCGGGATCACTA	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.6	57.7	1030-1050 bp	1.0	76.1
ycf15-rps12_p5	CTCGGTCCACAGAGACAAAA	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.5	57.7	1090-1110 bp	1.0	75.3