Hotspot

ndhD-psaC

SSC · 117062-117158 · 97 bp

Back to Pilosella

Pi 0.0247

MarkerSeek score 60.10

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 97
variable_sites: 6
pi: 0.0247
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AGGAACCCGCAAATATTGGT	ACAGAGGATTGTGTCGGTTG	45.0	50.0	57.5	57.8	287 bp	1.0	76.1
ndhD-psaC_p2	AGGAACCCGCAAATATTGGT	CAGAGGATTGTGTCGGTTGT	45.0	50.0	57.5	57.8	286 bp	1.0	76.1
ndhD-psaC_p3	AGGAACCCGCAAATATTGGT	AGAGATGTGAATCTGCCTGC	45.0	50.0	57.5	57.7	265 bp	1.0	75.0
ndhD-psaC_p4	CCAAGGAAAGTTGTTCGTGG	ACAGAGGATTGTGTCGGTTG	50.0	50.0	57.2	57.8	247 bp	1.0	74.4
ndhD-psaC_p5	CCAAGGAAAGTTGTTCGTGG	CAGAGGATTGTGTCGGTTGT	50.0	50.0	57.2	57.8	246 bp	1.0	74.4