Hotspot

rpl20-rps12

LSC · 69458-70223 · 766 bp

Back to Pilosella

Pi 0.0048

MarkerSeek score 57.80

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 766
variable_sites: 9
pi: 0.0048
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCTCCGAGCGATATATCCCC	CAGCGTCGAGGAACATGTAC	55.0	55.0	57.9	58.7	810-832 bp	1.0	76.9
rpl20-rps12_p2	TCTCCGAGCGATATATCCCC	AGCGTCGAGGAACATGTACT	55.0	50.0	57.9	58.8	809-831 bp	1.0	76.1
rpl20-rps12_p3	TCTCCGAGCGATATATCCCC	TCAGCGTCGAGGAACATGTA	55.0	50.0	57.9	58.8	811-833 bp	1.0	76.1
rpl20-rps12_p4	TCTCCGAGCGATATATCCCC	GCGTCGAGGAACATGTACTA	55.0	50.0	57.9	57.2	808-830 bp	1.0	76.0
rpl20-rps12_p5	TTCTACGTCTCCGAGCGATA	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.8	58.7	817-839 bp	1.0	75.4