Hotspot

psbE-petL

LSC · 65116-66442 · 1327 bp

Back to Pilosella

Pi 0.00456

MarkerSeek score 59.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1327
variable_sites: 15
pi: 0.00456
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCCGTCAGTAGAAAACCG	50.0	50.0	57.9	58.2	1426-1474 bp	1.0	81.7
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GCCGTCAGTAGAAAACCGAA	50.0	50.0	57.9	58.2	1424-1472 bp	1.0	81.6
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AAGCCGTCAGTAGAAAACCG	50.0	50.0	57.8	58.2	1349-1397 bp	1.0	81.0
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	GCCGTCAGTAGAAAACCGAA	50.0	50.0	57.8	58.2	1347-1395 bp	1.0	81.0
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AAGCCGTCAGTAGAAAACCG	45.0	50.0	58.2	58.2	1435-1483 bp	1.0	80.7