Hotspot

trnG-trnfM

LSC · 36385-36580 · 196 bp

Back to Pilosella

Pi 0.0163

MarkerSeek score 49.70

Primer available yes

Label trnG-trnfM

Feature Metadata

label_name: trnG-trnfM
feature_type: igs
region: LSC
length_bp: 196
variable_sites: 8
pi: 0.0163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnfM_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	273-274 bp	1.0	81.0
trnG-trnfM_p2	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.8	58.0	309-310 bp	1.0	80.8
trnG-trnfM_p3	TCTTTGCCAAGGAGAAGACG	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.8	58.1	267-268 bp	1.0	80.5
trnG-trnfM_p4	TCTTTGCCAAGGAGAAGACG	TCACGGGTTCAAATCCTGTC	50.0	50.0	57.8	57.8	266-267 bp	1.0	79.9
trnG-trnfM_p5	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	271-272 bp	1.0	79.9