Hotspot

atpI-atpH

LSC · 25316-26458 · 1143 bp

Back to Pilosella

Pi 0.0021

MarkerSeek score 53.20

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1143
variable_sites: 6
pi: 0.0021
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TCCCTGTCATGTTCCTTGGA	ATAACGGAAGCGGCAGAAAT	50.0	45.0	58.3	58.0	1275-1279 bp	1.0	78.2
atpI-atpH_p2	TCCCTGTCATGTTCCTTGGA	AGCCAATCCAGCAGCAATAA	50.0	45.0	58.3	57.9	1291-1295 bp	1.0	77.4
atpI-atpH_p3	TCCCTGTCATGTTCCTTGGA	AATAACGGAAGCGGCAGAAA	50.0	45.0	58.3	58.2	1276-1280 bp	1.0	77.1
atpI-atpH_p4	TCCCTGTCATGTTCCTTGGA	GCCAATCCAGCAGCAATAAC	50.0	50.0	58.3	57.8	1290-1294 bp	1.0	76.8
atpI-atpH_p5	TCCCTGTCATGTTCCTTGGA	TCCAGCAGCAATAACGGAAG	50.0	50.0	58.3	58.3	1285-1289 bp	1.0	76.5