Hotspot

ycf1

SSC · 122074-127347 · 5274 bp

Back to Pilea

Pi 0.0924
MarkerSeek score 78.00
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5274
variable_sites
1612
pi
0.0924
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TACATTTTACGACCTGGCGG GCCGTTCGGTATGATGGTTA 50.0 50.0 58.0 57.8 533 bp 0.1875 46.2
ycf1_p2 TACATTTTACGACCTGGCGG TATGCCGTTCGGTATGATGG 50.0 50.0 58.0 57.6 536 bp 0.1875 44.4
ycf1_p3 GGCCTTTTTCTTGGTCCCTT GCCGTTCGGTATGATGGTTA 50.0 50.0 58.3 57.8 920 bp 0.1875 44.1
ycf1_p4 ATGGCCTTTTTCTTGGTCCC GCCGTTCGGTATGATGGTTA 50.0 50.0 58.4 57.8 922 bp 0.1875 43.6
ycf1_p5 TGGCGGTTCGTGTATGTTTT GCCGTTCGGTATGATGGTTA 45.0 50.0 58.4 57.8 519 bp 0.1875 43.1