Hotspot

ndhF-rpl32

SSC · 110750-111781 · 1032 bp

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Pi 0.0921
MarkerSeek score 80.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1032
variable_sites
315
pi
0.0921
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CCGGGTGAACCAAAAACCTA AGACTGACTGCTTTTCGAATCA 50.0 40.9 58.0 58.0 1282-1845 bp 0.75 65.8
ndhF-rpl32_p2 TGTTGCCGTGGGAAAAAGTA AGACTGACTGCTTTTCGAATCA 45.0 40.9 57.9 58.0 1330-1440 bp 0.625 60.5
ndhF-rpl32_p3 TGTTGCCGTGGGAAAAAGTA AGTGGCTGCAAATTTCCTATCT 45.0 40.9 57.9 58.0 1823-1837 bp 0.1875 33.2
ndhF-rpl32_p4 CCGGGTGAACCAAAAACCTA AGTGGCTGCAAATTTCCTATCT 50.0 40.9 58.0 58.0 2228-2242 bp 0.1875 33.1
ndhF-rpl32_p5 CCGGGTGAACCAAAAACCTA GTGGCTGCAAATTTCCTATCTG 50.0 45.5 58.0 58.0 2227-2241 bp 0.1875 33.0