Hotspot

rbcL-accD

LSC · 55898-56650 · 753 bp

Back to Pilea

Pi 0.0517
MarkerSeek score 84.20
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
753
variable_sites
147
pi
0.0517
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GGCTTCAGGGGGTATTCATG ATAGGATCCCAAGTACCCGG 55.0 55.0 58.0 58.0 1791-1833 bp 0.9375 87.0
rbcL-accD_p2 AATGAGGGACGTGATCTTGC ATAGGATCCCAAGTACCCGG 50.0 55.0 58.0 58.0 1628-1670 bp 0.9375 87.0
rbcL-accD_p3 GCCGTAGCTAATCGAGTAGC ATAGGATCCCAAGTACCCGG 55.0 55.0 58.0 58.0 1673-1712 bp 0.75 79.3
rbcL-accD_p4 GGCTTCAGGGGGTATTCATG TGGGCCACACCTGTATTCTA 55.0 50.0 58.0 58.0 1108-1114 bp 0.1875 48.8
rbcL-accD_p5 TCGGTGGAGGAACTTTAGGT ATAGGATCCCAAGTACCCGG 50.0 55.0 58.0 58.0 1720-1726 bp 0.1875 48.4