Hotspot

ycf3-trnS-GCU

LSC · 44181-44802 · 622 bp

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Pi 0.0553
MarkerSeek score 82.00
Primer available yes
Label ycf3-trnS-GCU

Feature Metadata

label_name
ycf3-trnS-GCU
feature_type
igs
region
LSC
length_bp
622
variable_sites
117
pi
0.0553
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.95
markerseek_score
82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GCU_p1 ACTTCACCTTCAACAAGCGT AAGAAAGGTGCCCGTGATTT 45.0 45.0 58.0 58.0 1062-1069 bp 0.1875 49.1
ycf3-trnS-GCU_p2 CCAACCCTCTTGGAAAAGCT AAGAAAGGTGCCCGTGATTT 50.0 45.0 58.0 58.0 1490-1498 bp 0.1875 49.0
ycf3-trnS-GCU_p3 CCAACCCTCTTGGAAAAGCT GGTGCCCGTGATTTGATTTG 50.0 50.0 58.0 58.0 1484-1492 bp 0.1875 49.0
ycf3-trnS-GCU_p4 TATTTGTCTTAAGCGCCGCT AAGAAAGGTGCCCGTGATTT 45.0 45.0 58.0 58.0 1413-1419 bp 0.1875 48.8
ycf3-trnS-GCU_p5 TATTTGTCTTAAGCGCCGCT GGTGCCCGTGATTTGATTTG 45.0 50.0 58.0 58.0 1407-1413 bp 0.1875 48.8