Hotspot

psbM-trnD-GUC

LSC · 29428-29922 · 495 bp

Back to Pilea

Pi 0.0677
MarkerSeek score 82.10
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
495
variable_sites
117
pi
0.0677
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.97
markerseek_score
82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 ACCTACCCCCTTTTAGTCCT TAGTATGATGGCGGTTGTGC 50.0 50.0 56.9 58.1 1801-1814 bp 0.1875 40.0
psbM-trnD-GUC_p2 ACCTACCCCCTTTTAGTCCT AAGTATGCCCCCATCGTCTA 50.0 50.0 56.9 57.9 1781-1794 bp 0.1875 39.6
psbM-trnD-GUC_p3 ACCTACCCCCTTTTAGTCCT TAGCGGTTCAGGACATCTCT 50.0 50.0 56.9 57.9 1763-1776 bp 0.1875 39.3
psbM-trnD-GUC_p4 ACCTACCCCCTTTTAGTCCT TCTAGCGGTTCAGGACATCT 50.0 50.0 56.9 57.9 1765-1778 bp 0.1875 39.3
psbM-trnD-GUC_p5 ACCTACCCCCTTTTAGTCCT GTCTAGCGGTTCAGGACATC 50.0 55.0 56.9 57.8 1766-1779 bp 0.1875 38.7