Hotspot

matK-trnK-UUU

LSC · 3378-4139 · 762 bp

Back to Pilea

Pi 0.0528
MarkerSeek score 82.80
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
762
variable_sites
136
pi
0.0528
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.99
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 GGCATTTCCACAATGACTGC CTTTGATTTGATTGCGCCGT 50.0 45.0 58.0 58.0 1203-1210 bp 0.1875 48.2
matK-trnK-UUU_p2 TGAGAAGATTCGTTGCGGAG CTTTGATTTGATTGCGCCGT 50.0 45.0 58.0 58.0 1581-1588 bp 0.1875 48.1
matK-trnK-UUU_p3 GTCGTAGGGAACATGGCATT CTTTGATTTGATTGCGCCGT 50.0 45.0 58.0 58.0 1217-1224 bp 0.1875 47.9
matK-trnK-UUU_p4 GGCATTTCCACAATGACTGC CGCTTCGATATGTAGTGCCA 50.0 50.0 58.0 57.9 1540-1556 bp 0.1875 47.7
matK-trnK-UUU_p5 TCTCTGCCCGTTAAGGAAGA CTTTGATTTGATTGCGCCGT 50.0 45.0 58.1 58.0 1242-1249 bp 0.1875 47.6