Hotspot

trnV-GAC-rps12

Genome · 111698-113248 · 1551 bp

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Pi 0.00458
MarkerSeek score 56.00
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
Genome
length_bp
1551
variable_sites
25
pi
0.00458
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 TGGAAGGGCTCACATTAGGT TGGCAACTTTACGTAAGGCA 50.0 45.0 58.3 57.7 1506-1700 bp 1.0 80.5
trnV-GAC-rps12_p2 GTCAAGGTGACACTCTACCG TGGCAACTTTACGTAAGGCA 55.0 45.0 57.7 57.7 1448-1642 bp 1.0 80.4
trnV-GAC-rps12_p3 GGCTCGAACTGATGACTTCC TGGCAACTTTACGTAAGGCA 55.0 45.0 58.4 57.7 1473-1667 bp 1.0 80.3
trnV-GAC-rps12_p4 GTTTGATGGAAGGGCTCACA TGGCAACTTTACGTAAGGCA 50.0 45.0 58.1 57.7 1512-1704 bp 0.916667 78.9
trnV-GAC-rps12_p5 TGTTTGATGGAAGGGCTCAC TGGCAACTTTACGTAAGGCA 50.0 45.0 58.1 57.7 1513-1705 bp 0.916667 78.9