Hotspot

trnI-CAU-trnF-GAA

Genome · 67827-69662 · 1836 bp

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Pi 0.00485
MarkerSeek score 55.20
Primer available yes
Label trnI-CAU-trnF-GAA

Feature Metadata

label_name
trnI-CAU-trnF-GAA
feature_type
igs
region
Genome
length_bp
1836
variable_sites
29
pi
0.00485
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU-trnF-GAA_p1 GTTGGGCGCTTTAACCATTC AGGACTGAAAATCCTCGTGC 50.0 50.0 58.0 57.9 1914-1919 bp 1.0 80.5
trnI-CAU-trnF-GAA_p2 GTTGGGCGCTTTAACCATTC GACTGAAAATCCTCGTGCCA 50.0 50.0 58.0 58.2 1912-1917 bp 1.0 79.8
trnI-CAU-trnF-GAA_p3 GTTGGGCGCTTTAACCATTC ACCCTGTACCTGGATGATCC 50.0 55.0 58.0 58.2 1971-1976 bp 1.0 79.7
trnI-CAU-trnF-GAA_p4 GTTGGGCGCTTTAACCATTC CCCTGTACCTGGATGATCCA 50.0 55.0 58.0 58.2 1970-1975 bp 1.0 79.7
trnI-CAU-trnF-GAA_p5 CCCGCGAGTTCACCAATTAT AGGACTGAAAATCCTCGTGC 50.0 50.0 58.3 57.9 1936-1941 bp 1.0 77.9