Hotspot

trnT-GGU-psaM

Genome · 30644-31904 · 1261 bp

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Pi 0.00576
MarkerSeek score 55.40
Primer available yes
Label trnT-GGU-psaM

Feature Metadata

label_name
trnT-GGU-psaM
feature_type
igs
region
Genome
length_bp
1261
variable_sites
25
pi
0.00576
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.98
markerseek_score
55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psaM_p1 GAACCGATGACTTACGCCTT GCCAGGCAGGTCAGAAAAA 50.0 52.6 58.0 58.3 1393-1436 bp 1.0 74.2
trnT-GGU-psaM_p2 TACCGCTGAGTTAAAAGGGC GCCAGGCAGGTCAGAAAAA 50.0 52.6 57.9 58.3 1358-1401 bp 1.0 73.6
trnT-GGU-psaM_p3 CCGATGACTTACGCCTTACC GCCAGGCAGGTCAGAAAAA 55.0 52.6 58.2 58.3 1390-1433 bp 1.0 73.1
trnT-GGU-psaM_p4 TTGAACCGATGACTTACGCC GCCAGGCAGGTCAGAAAAA 50.0 52.6 58.3 58.3 1395-1438 bp 1.0 72.0
trnT-GGU-psaM_p5 CGATGACTTACGCCTTACCA GCCAGGCAGGTCAGAAAAA 50.0 52.6 57.4 58.3 1389-1432 bp 1.0 69.7