Hotspot

psbJ-petA

Genome · 15399-16444 · 1046 bp

Back to Picea

Pi 0.00554
MarkerSeek score 44.80
Primer available yes
Label psbJ-petA

Feature Metadata

label_name
psbJ-petA
feature_type
igs
region
Genome
length_bp
1046
variable_sites
18
pi
0.00554
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbJ-petA_p1 GGGAGTAAGAGAAATGGCCG TGTGGGTGGATTTGGTCAAG 55.0 50.0 58.0 58.0 1333-1347 bp 1.0 81.5
psbJ-petA_p2 TGGAAGGATCCCTCTTTGGT TGTGGGTGGATTTGGTCAAG 50.0 50.0 57.9 58.0 1306-1320 bp 1.0 81.4
psbJ-petA_p3 TGGCTTATGATGTGTTCGGG TGTGGGTGGATTTGGTCAAG 50.0 50.0 58.0 58.0 1845-1859 bp 1.0 81.2
psbJ-petA_p4 AATGCAGTTCATCCAGCGAT TGTGGGTGGATTTGGTCAAG 45.0 50.0 58.0 58.0 1610-1624 bp 1.0 81.2
psbJ-petA_p5 CCGGCCGAATGAGTATTTCA TGTGGGTGGATTTGGTCAAG 50.0 50.0 58.1 58.0 1820-1834 bp 1.0 81.1