Pi
0.0139
MarkerSeek score
60.50
Primer available
yes
Label
trnV(GAC)
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnV(GAC)_p1 | TAGTGGCGTTGAGTTTCTCG | TCAATCCCACGAGCCTCTTA | 50.0 | 50.0 | 57.9 | 58.1 | 221 bp | 1.0 | 78.8 |
| trnV(GAC)_p2 | CGTTGAGTTTCTCGACCCTT | TCAATCCCACGAGCCTCTTA | 50.0 | 50.0 | 57.8 | 58.1 | 215 bp | 1.0 | 78.1 |
| trnV(GAC)_p3 | CTATTTCTCGATGGGGGCAG | TCAATCCCACGAGCCTCTTA | 55.0 | 50.0 | 57.8 | 58.1 | 175 bp | 1.0 | 77.9 |
| trnV(GAC)_p4 | TCTATTTCTCGATGGGGGCA | TCAATCCCACGAGCCTCTTA | 50.0 | 50.0 | 58.2 | 58.1 | 176 bp | 1.0 | 77.7 |
| trnV(GAC)_p5 | ATAGTGCGAAAAGAAGGCCC | TCAATCCCACGAGCCTCTTA | 50.0 | 50.0 | 58.2 | 58.1 | 261 bp | 1.0 | 77.2 |
| trnV(GAC)_p1 | TCAATCCCACGAGCCTCTTA | TAGTGGCGTTGAGTTTCTCG | 50.0 | 50.0 | 58.1 | 57.9 | 221 bp | 1.0 | 78.8 |
| trnV(GAC)_p2 | TCAATCCCACGAGCCTCTTA | CGTTGAGTTTCTCGACCCTT | 50.0 | 50.0 | 58.1 | 57.8 | 215 bp | 1.0 | 78.1 |
| trnV(GAC)_p3 | TCAATCCCACGAGCCTCTTA | CTATTTCTCGATGGGGGCAG | 50.0 | 55.0 | 58.1 | 57.8 | 175 bp | 1.0 | 77.9 |
| trnV(GAC)_p4 | TCAATCCCACGAGCCTCTTA | TCTATTTCTCGATGGGGGCA | 50.0 | 50.0 | 58.1 | 58.2 | 176 bp | 1.0 | 77.7 |
| trnV(GAC)_p5 | TCAATCCCACGAGCCTCTTA | ATAGTGCGAAAAGAAGGCCC | 50.0 | 50.0 | 58.1 | 58.2 | 261 bp | 1.0 | 77.2 |