Hotspot

rpl32-trnL(UAG)

SSC · 114961-115502 · 542 bp

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Pi 0.0185

MarkerSeek score 56.20

Primer available yes

Label rpl32-trnL(UAG)

Feature Metadata

label_name: rpl32-trnL(UAG)
feature_type: igs
region: SSC
length_bp: 542
variable_sites: 10
pi: 0.0185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL(UAG)_p1	GTTGCCGTCGGAAAAAGAAG	AAAAATAGCACTTGGGCCGA	50.0	45.0	58.0	58.1	1768-1769 bp	1.0	80.0
rpl32-trnL(UAG)_p2	GTTGCCGTCGGAAAAAGAAG	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	58.0	58.1	1788-1789 bp	1.0	80.0
rpl32-trnL(UAG)_p3	GTTGCCGTCGGAAAAAGAAG	TGAAGTAGCAAAGCCTTGGG	50.0	50.0	58.0	58.1	1789-1790 bp	1.0	80.0
rpl32-trnL(UAG)_p4	TGCCGTCGGAAAAAGAAGAA	AAAAATAGCACTTGGGCCGA	45.0	45.0	58.0	58.1	1766-1767 bp	1.0	79.9
rpl32-trnL(UAG)_p5	TTGCCGTCGGAAAAAGAAGA	AAAAATAGCACTTGGGCCGA	45.0	45.0	58.0	58.1	1767-1768 bp	1.0	79.9