Hotspot

rpoB-trnC(GCA)

LSC · 27963-29143 · 1181 bp

Back to Picconia

Pi 0.00593

MarkerSeek score 56.20

Primer available yes

Label rpoB-trnC(GCA)

Feature Metadata

label_name: rpoB-trnC(GCA)
feature_type: igs
region: LSC
length_bp: 1181
variable_sites: 7
pi: 0.00593
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC(GCA)_p1	CTCATTTCCATCCCCGAGC	CCGGATTTGAACTGGGGAAA	57.9	50.0	58.0	58.1	1264-1269 bp	1.0	71.7
rpoB-trnC(GCA)_p2	ATTCCCTCATTTCCATCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.9	58.1	1269-1274 bp	1.0	71.1
rpoB-trnC(GCA)_p3	CTCATTTCCATCCCCGAGC	AAAGGATTTGCAGTCCTCCG	57.9	50.0	58.0	58.2	1245-1250 bp	1.0	71.0
rpoB-trnC(GCA)_p4	TCATTTCCATCCCCGAGCAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	59.2	58.1	1263-1268 bp	1.0	70.6
rpoB-trnC(GCA)_p5	ATTCCCTCATTTCCATCCCC	AAAGGATTTGCAGTCCTCCG	50.0	50.0	56.9	58.2	1250-1255 bp	1.0	70.4