Hotspot

matK-rps16

LSC · 3664-5305 · 1642 bp

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Pi 0.00549

MarkerSeek score 51.50

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 1642
variable_sites: 9
pi: 0.00549
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	CCCTGATCATGAGCAAGTCC	GGAACTTCGACCTAATCAAACG	55.0	45.5	58.0	57.4	1779-1783 bp	1.0	59.7
matK-rps16_p2	CCTGATCATGAGCAAGTCCA	GGAACTTCGACCTAATCAAACG	50.0	45.5	57.3	57.4	1778-1782 bp	1.0	54.3
matK-rps16_p3	ACCCTGATCATGAGCAAGTC	GGAACTTCGACCTAATCAAACG	50.0	45.5	57.3	57.4	1780-1784 bp	1.0	54.3
matK-rps16_p4	CCCTGATCATGAGCAAGTCC	GAACTTCGACCTAATCAAACGA	55.0	40.9	58.0	56.5	1778-1782 bp	1.0	52.3
matK-rps16_p5	CCCTGATCATGAGCAAGTCC	ACTTCGACCTAATCAAACGA	55.0	40.0	58.0	54.5	1776-1780 bp	1.0	52.0