Hotspot

ccsA-ndhD

SSC · 116750-116948 · 199 bp

Back to Phytolacca

Pi 0.0433

MarkerSeek score 62.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 199
variable_sites: 20
pi: 0.0433
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGTTCATTTACATTAACGCT	TCAGTTGAGAAAGTGGAAGCT	33.3	42.9	53.8	57.2	275 bp	1.0	45.8
ccsA-ndhD_p2	TGGTTCATTTACATTAACGCTT	TCAGTTGAGAAAGTGGAAGCT	31.8	42.9	54.7	57.2	275 bp	1.0	45.8
ccsA-ndhD_p3	ATGGTTCATTTACATTAACGCT	TCAGTTGAGAAAGTGGAAGCT	31.8	42.9	54.4	57.2	276 bp	1.0	45.8
ccsA-ndhD_p4	TGGTTCATTTACATTAACGCT	ATCAGTTGAGAAAGTGGAAGCT	33.3	40.9	53.8	57.6	276 bp	1.0	45.8
ccsA-ndhD_p5	TGGTTCATTTACATTAACGCT	TCAGTTGAGAAAGTGGAAGC	33.3	45.0	53.8	55.6	275 bp	1.0	45.8