Hotspot

rpl32-trnL-UAG

SSC · 114403-115581 · 1179 bp

Back to Phytolacca

Pi 0.0267

MarkerSeek score 65.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1179
variable_sites: 80
pi: 0.0267
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAAGGATATTGGGCAGCGTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.9	57.9	1305-1350 bp	1.0	85.4
rpl32-trnL-UAG_p2	AAAAGGATATTGGGCAGCGT	ATCCGCATAAAAGAGCTGCA	45.0	45.0	57.9	58.0	1927-1970 bp	1.0	84.4
rpl32-trnL-UAG_p3	AAAAGGATATTGGGCAGCGT	TCCGCATAAAAGAGCTGCAT	45.0	45.0	57.9	58.0	1926-1969 bp	1.0	84.4
rpl32-trnL-UAG_p4	AAAGGATATTGGGCAGCGTT	ATCCGCATAAAAGAGCTGCA	45.0	45.0	57.9	58.0	1926-1969 bp	1.0	84.4
rpl32-trnL-UAG_p5	AAAGGATATTGGGCAGCGTT	TCCGCATAAAAGAGCTGCAT	45.0	45.0	57.9	58.0	1925-1968 bp	1.0	84.4