Hotspot

ndhF-rpl32

SSC · 113049-114240 · 1192 bp

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Pi 0.0308
MarkerSeek score 72.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1192
variable_sites
90
pi
0.0308
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTTTGTTGCCGTCGGAAAAA AACGCTGCCCAATATCCTTT 40.0 45.0 57.4 57.9 1321-1401 bp 1.0 82.9
ndhF-rpl32_p2 TTTTGTTGCCGTCGGAAAAA ACGCTGCCCAATATCCTTTT 40.0 45.0 57.4 57.9 1320-1400 bp 1.0 82.9
ndhF-rpl32_p3 TTTTTGTTGCCGTCGGAAAA AACGCTGCCCAATATCCTTT 40.0 45.0 57.4 57.9 1322-1402 bp 1.0 82.9
ndhF-rpl32_p4 TTTTTGTTGCCGTCGGAAAA ACGCTGCCCAATATCCTTTT 40.0 45.0 57.4 57.9 1321-1401 bp 1.0 82.9
ndhF-rpl32_p5 TTTTTGTTGCCGTCGGAAAAA ACGCTGCCCAATATCCTTTT 38.1 45.0 58.0 57.9 1321-1401 bp 1.0 79.7